| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Valentino Rossi | Movistar Yamaha | 340.3 | 01:46.504 | ||||
| 2 | Maverick Viñales | Suzuki Ecstar | 339.6 | 01:46.598 | 0.094 / 0.094 | |||
| 3 | Andrea Iannone | Ducati | 347.3 | 01:46.607 | 0.103 / 0.009 | |||
| 4 | Marc Marquez | Repsol Honda | 341.2 | 01:46.759 | 0.255 / 0.152 | |||
| 5 | Jorge Lorenzo | Movistar Yamaha | 340.4 | 01:46.882 | 0.378 / 0.123 | |||
| 6 | Aleix Espargaro+ | Suzuki Ecstar | 339.9 | 01:47.186 | 0.682 / 0.304 | |||
| 7 | Dani Pedrosa | Repsol Honda | 341.6 | 01:47.218 | 0.714 / 0.032 | |||
| 8 | Bradley Smith | Tech 3 | 338.8 | 01:47.247 | 0.743 / 0.029 | |||
| 9 | Danilo Petrucci | Octo Pramac Yakhnich | 349.4 | 01:47.261 | 0.757 / 0.014 | |||
| 10 | Scott Redding+ | Octo Pramac Yakhnich | 345.2 | 01:47.359 | 0.855 / 0.098 | |||
| 11 | Michele Pirro* | Ducati | 346.4 | 01:47.361 | 0.857 / 0.002 | |||
| 12 | Yonny Hernandez | Aspar Team | 346.0 | 01:47.436 | 0.932 / 0.075 |
+Riders advanced from Q1 to Q2
| 13 | Andrea Dovizioso | Ducati | 348.1 | 01:47.089 | 0.203 / 0.049 | |||
| 14 | Pol Espargaro | Tech 3 | 338.9 | 01:47.159 | 0.273 / 0.070 | |||
| 15 | Hector Barbera | Avintia Racing | 342.6 | 01:47.555 | 0.669 / 0.396 | |||
| 16 | Cal Crutchlow | LCR Honda | 334.9 | 01:47.659 | 0.773 / 0.104 | |||
| 17 | Jack Miller | EG 0,0 Marc VDS | 334.5 | 01:47.830 | 0.944 / 0.171 | |||
| 18 | Eugene Laverty | Aspar Team | 345.4 | 01:48.111 | 1.225 / 0.281 | |||
| 19 | Alvaro Bautista | Aprilia Gresini | 330.6 | 01:48.372 | 1.486 / 0.261 | |||
| 20 | Stefan Bradl | Aprilia Gresini | 335.7 | 01:48.646 | 1.760 / 0.274 | |||
| 21 | Loris Baz | Avintia Racing | 338.8 | 01:48.991 | 2.105 / 0.345 |
*Wild card entry
Note: Team Estrella Galicia 0,0 Marc VDS rider Tito Rabat was forced to withdraw from the Italian Grand Prix after sustaining a collarbone fracture in FP3