MotoGP Practice Results Brno Czech Republic Friday June 19
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Ai Ogura | Trackhouse Racing | 324.3 | 01:51.735 | |
| 2 | Marco Bezzecchi | Aprilia Racing | 328.2 | 01:51.826 | 0.091 / 0.091 |
| 3 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 323.3 | 01:51.942 | 0.207 / 0.116 |
| 4 | Francesco Bagnaia | Ducati Lenovo | 326.2 | 01:51.983 | 0.248 / 0.041 |
| 5 | Marc Marquez | Ducati Lenovo | 323.3 | 01:51.988 | 0.253 / 0.005 |
| 6 | Pedro Acosta | Red Bull KTM | 324.3 | 01:52.228 | 0.493 / 0.240 |
| 7 | Joan Mir | Honda HRC Castrol | 322.3 | 01:52.253 | 0.518 / 0.025 |
| 8 | Diogo Moreira | Pro Honda LCR | 321.4 | 01:52.315 | 0.580 / 0.062 |
| 9 | Fermin Aldeguer | Gresini Racing | 319.5 | 01:52.320 | 0.585 / 0.005 |
| 10 | Raul Fernandez | Trackhouse Racing | 319.5 | 01:52.354 | 0.619 / 0.034 |
| 11 | Jorge Martin | Aprilia Racing | 321.4 | 01:52.365 | 0.630 / 0.011 |
| 12 | Maverick Viñales | KTM Tech3 | 323.3 | 01:52.392 | 0.657 / 0.027 |
| 13 | Luca Marini | Honda HRC Castrol | 321.4 | 01:52.424 | 0.689 / 0.032 |
| 14 | Fabio Quartararo | Monster Energy Yamaha | 319.5 | 01:52.533 | 0.798 / 0.109 |
| 15 | Alex Marquez | Gresini Racing | 324.3 | 01:52.556 | 0.821 / 0.023 |
| 16 | Enea Bastianini | KTM Tech3 | 322.3 | 01:52.561 | 0.826 / 0.005 |
| 17 | Franco Morbidelli | Pertamina Enduro VR46 | 321.4 | 01:52.576 | 0.841 / 0.015 |
| 18 | Jack Miller | Pramac Racing | 320.4 | 01:52.583 | 0.848 / 0.007 |
| 19 | Brad Binder | Red Bull KTM | 325.3 | 01:52.599 | 0.864 / 0.016 |
| 20 | Alex Rins | Monster Energy Yamaha | 321.4 | 01:53.020 | 1.285 / 0.421 |
| 21 | Toprak Razgatlıoğlu | Pramac Racing | 321.4 | 01:53.398 | 1.663 / 0.378 |
| 22 | Cal Crutchlow* | Castrol Honda LCR | 321.4 | 01:53.847 | 2.112 / 0.449 |
*Replaced Johann Zarco for the Czech Republic Grand Prix
Note: Castrol Honda LCR rider Johann Zarco (fibula fracture recovery) was ruled out of the Czech Republic Grand Prix.