MotoGP Qualifying Results Brno Czech Republic Saturday June 20
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Ai Ogura | Trackhouse Racing | 325.3 | 01:51.139 | |
| 2 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 321.4 | 01:51.350 | 0.211 / 0.211 |
| 3 | Francesco Bagnaia | Ducati Lenovo | 324.3 | 01:51.383 | 0.244 / 0.033 |
| 4 | Marco Bezzecchi | Aprilia Racing | 322.3 | 01:51.428 | 0.289 / 0.045 |
| 5 | Marc Marquez | Ducati Lenovo | 323.3 | 01:51.436 | 0.297 / 0.008 |
| 6 | Diogo Moreira | Pro Honda LCR | 324.3 | 01:51.691 | 0.552 / 0.255 |
| 7 | Raul Fernandez | Trackhouse Racing | 323.3 | 01:51.772 | 0.633 / 0.081 |
| 8 | Pedro Acosta | Red Bull KTM | 324.3 | 01:51.821 | 0.682 / 0.049 |
| 9 | Franco Morbidelli+ | Pertamina Enduro VR46 | 321.4 | 01:51.851 | 0.712 / 0.030 |
| 10 | Jorge Martin+ | Aprilia Racing | 324.3 | 01:51.909 | 0.770 / 0.058 |
| 11 | Fermin Aldeguer | Gresini Racing | 320.4 | 01:52.044 | 0.905 / 0.135 |
| 12 | Joan Mir | Honda HRC Castrol | 323.3 | 01:52.084 | 0.945 / 0.040 |
+Riders advanced from Q1 to Q2
| 13 | Maverick Viñales | KTM Tech3 | 321.4 | 01:52.053 | 0.234 / 0.033 |
| 14 | Alex Marquez | Gresini Racing | 326.2 | 01:52.086 | 0.267 / 0.033 |
| 15 | Fabio Quartararo | Monster Energy Yamaha | 317.6 | 01:52.185 | 0.366 / 0.099 |
| 16 | Luca Marini | Honda HRC Castrol | 323.3 | 01:52.263 | 0.444 / 0.078 |
| 17 | Enea Bastianini | KTM Tech3 | 327.2 | 01:52.357 | 0.538 / 0.094 |
| 18 | Jack Miller | Pramac Racing | 321.4 | 01:52.420 | 0.601 / 0.063 |
| 19 | Alex Rins | Monster Energy Yamaha | 322.3 | 01:52.506 | 0.687 / 0.086 |
| 20 | Brad Binder | Red Bull KTM | 325.3 | 01:52.556 | 0.737 / 0.050 |
| 21 | Toprak Razgatlıoğlu | Pramac Racing | 321.4 | 01:52.626 | 0.807 / 0.070 |
| 22 | Cal Crutchlow* | Castrol Honda LCR | 322.3 | 01:53.599 | 1.780 / 0.973 |
*Replaces Johann Zarco for the Czech Republic Grand Prix
Note: Castrol Honda LCR rider Johann Zarco (fibula fracture recovery) was ruled out of the Czech Republic Grand Prix.