MotoGP Second Free Practice Results Brno Czech Republic Saturday June 20
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Raul Fernandez | Trackhouse Racing | 316.7 | 01:52.050 | |
| 2 | Marc Marquez | Ducati Lenovo | 323.3 | 01:52.885 | 0.835 / 0.835 |
| 3 | Maverick Viñales | KTM Tech3 | 321.4 | 01:52.915 | 0.865 / 0.030 |
| 4 | Fermin Aldeguer | Gresini Racing | 321.4 | 01:53.012 | 0.962 / 0.097 |
| 5 | Ai Ogura | Trackhouse Racing | 324.3 | 01:53.021 | 0.971 / 0.009 |
| 6 | Pedro Acosta | Red Bull KTM | 323.3 | 01:53.043 | 0.993 / 0.022 |
| 7 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 323.3 | 01:53.084 | 1.034 / 0.041 |
| 8 | Marco Bezzecchi | Aprilia Racing | 324.3 | 01:53.103 | 1.053 / 0.019 |
| 9 | Diogo Moreira | Pro Honda LCR | 323.3 | 01:53.188 | 1.138 / 0.085 |
| 10 | Joan Mir | Honda HRC Castrol | 322.3 | 01:53.190 | 1.140 / 0.002 |
| 11 | Fabio Quartararo | Monster Energy Yamaha | 319.5 | 01:53.256 | 1.206 / 0.066 |
| 12 | Franco Morbidelli | Pertamina Enduro VR46 | 320.4 | 01:53.395 | 1.345 / 0.139 |
| 13 | Luca Marini | Honda HRC Castrol | 320.4 | 01:53.444 | 1.394 / 0.049 |
| 14 | Jorge Martin | Aprilia Racing | 323.3 | 01:53.445 | 1.395 / 0.001 |
| 15 | Alex Marquez | Gresini Racing | 323.3 | 01:53.464 | 1.414 / 0.019 |
| 16 | Francesco Bagnaia | Ducati Lenovo | 325.3 | 01:53.492 | 1.442 / 0.028 |
| 17 | Toprak Razgatlıoğlu | Pramac Racing | 320.4 | 01:53.496 | 1.446 / 0.004 |
| 18 | Enea Bastianini | KTM Tech3 | 325.3 | 01:53.563 | 1.513 / 0.067 |
| 19 | Brad Binder | Red Bull KTM | 324.3 | 01:53.649 | 1.599 / 0.086 |
| 20 | Jack Miller | Pramac Racing | 318.5 | 01:53.815 | 1.765 / 0.166 |
| 21 | Alex Rins | Monster Energy Yamaha | 318.5 | 01:53.979 | 1.929 / 0.164 |
| 22 | Cal Crutchlow* | Castrol Honda LCR | 317.6 | 01:54.723 | 2.673 / 0.744 |
*Replaces Johann Zarco for the Czech Republic Grand Prix
Note: Castrol Honda LCR rider Johann Zarco (fibula fracture recovery) was ruled out of the Czech Republic Grand Prix.