MotoGP Second Free Practice Results Mugello Italy Saturday May 30
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 356.4 | 01:45.641 | |
| 2 | Pedro Acosta | Red Bull KTM | 357.6 | 01:45.885 | 0.244 / 0.244 |
| 3 | Francesco Bagnaia | Ducati Lenovo | 358.8 | 01:45.911 | 0.270 / 0.026 |
| 4 | Fermin Aldeguer | Gresini Racing | 354.0 | 01:45.942 | 0.301 / 0.031 |
| 5 | Ai Ogura | Trackhouse Racing | 366.1 | 01:45.988 | 0.347 / 0.046 |
| 6 | Marco Bezzecchi | Aprilia Racing | 364.8 | 01:46.070 | 0.429 / 0.082 |
| 7 | Franco Morbidelli | Pertamina Enduro VR46 | 351.7 | 01:46.086 | 0.445 / 0.016 |
| 8 | Jorge Martin | Aprilia Racing | 368.6 | 01:46.104 | 0.463 / 0.018 |
| 9 | Marc Marquez | Ducati Lenovo | 361.2 | 01:46.131 | 0.490 / 0.027 |
| 10 | Diogo Moreira | Pro Honda LCR | 362.4 | 01:46.150 | 0.509 / 0.019 |
| 11 | Raul Fernandez | Trackhouse Racing | 356.4 | 01:46.353 | 0.712 / 0.203 |
| 12 | Brad Binder | Red Bull KTM | 361.2 | 01:46.403 | 0.762 / 0.050 |
| 13 | Luca Marini | Honda HRC Castrol | 362.4 | 01:46.484 | 0.843 / 0.081 |
| 14 | Joan Mir | Honda HRC Castrol | 356.4 | 01:46.495 | 0.854 / 0.011 |
| 15 | Maverick Viñales | KTM Tech3 | 355.2 | 01:46.660 | 1.019 / 0.165 |
| 16 | Alex Rins | Monster Energy Yamaha | 350.6 | 01:46.745 | 1.104 / 0.085 |
| 17 | Enea Bastianini | KTM Tech3 | 364.8 | 01:46.801 | 1.160 / 0.056 |
| 18 | Fabio Quartararo | Monster Energy Yamaha | 354.0 | 01:46.844 | 1.203 / 0.043 |
| 19 | Jack Miller | Pramac Racing | 351.7 | 01:46.997 | 1.356 / 0.153 |
| 20 | Toprak Razgatlıoğlu | Pramac Racing | 352.9 | 01:47.341 | 1.700 / 0.344 |
| 21 | Michele Pirro* | Gresini Racing | 350.6 | 01:47.646 | 2.005 / 0.305 |
| 22 | Cal Crutchlow^ | Castrol Honda LCR | 351.7 | 01:48.550 | 2.909 / 0.904 |
*Replaced Alex Marquez for the Italian Grand Prix
^Replaced Johann Zarco for the Italian Grand Prix
Note: Gresini Racing rider Alex Marquez (collarbone injury recovery) and Castrol Honda LCR's Johann Zarco (fibula fracture recovery) were ruled out of the Italian Grand Prix.