MotoGP Second Free Practice Results Assen Netherlands Saturday June 27
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Marco Bezzecchi | Aprilia Racing | 312.1 | 01:31.611 | |
| 2 | Raul Fernandez | Trackhouse Racing | 305.0 | 01:31.731 | 0.120 / 0.120 |
| 3 | Ai Ogura | Trackhouse Racing | 307.6 | 01:31.850 | 0.239 / 0.119 |
| 4 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 309.4 | 01:31.920 | 0.309 / 0.070 |
| 5 | Marc Marquez | Ducati Lenovo | 309.4 | 01:31.990 | 0.379 / 0.070 |
| 6 | Pedro Acosta | Red Bull KTM | 309.4 | 01:32.113 | 0.502 / 0.123 |
| 7 | Jorge Martin | Aprilia Racing | 311.2 | 01:32.120 | 0.509 / 0.007 |
| 8 | Francesco Bagnaia | Ducati Lenovo | 310.3 | 01:32.191 | 0.580 / 0.071 |
| 9 | Alex Marquez | Gresini Racing | 312.1 | 01:32.337 | 0.726 / 0.146 |
| 10 | Franco Morbidelli | Pertamina Enduro VR46 | 309.4 | 01:32.356 | 0.745 / 0.019 |
| 11 | Enea Bastianini | KTM Tech3 | 311.2 | 01:32.366 | 0.755 / 0.010 |
| 12 | Fabio Quartararo | Monster Energy Yamaha | 301.6 | 01:32.391 | 0.780 / 0.025 |
| 13 | Luca Marini | Honda HRC Castrol | 311.2 | 01:32.502 | 0.891 / 0.111 |
| 14 | Diogo Moreira | Pro Honda LCR | 310.3 | 01:32.684 | 1.073 / 0.182 |
| 15 | Toprak Razgatlıoğlu | Pramac Racing | 305.0 | 01:32.686 | 1.075 / 0.002 |
| 16 | Maverick Viñales | KTM Tech3 | 308.5 | 01:32.776 | 1.165 / 0.090 |
| 17 | Alex Rins | Monster Energy Yamaha | 306.8 | 01:32.790 | 1.179 / 0.014 |
| 18 | Joan Mir | Honda HRC Castrol | 305.9 | 01:32.797 | 1.186 / 0.007 |
| 19 | Brad Binder | Red Bull KTM | 311.2 | 01:33.012 | 1.401 / 0.215 |
| 20 | Jack Miller | Pramac Racing | 305.0 | 01:33.289 | 1.678 / 0.277 |
| 21 | Augusto Fernandez* | Yamaha Factory Racing | 303.3 | 01:33.998 | 2.387 / 0.709 |
| 22 | Cal Crutchlow^ | Castrol Honda LCR | 301.6 | 01:34.519 | 2.908 / 0.521 |
*Wild card entry
^Replaced Johann Zarco for the Dutch Grand Prix
Note: Gresini Racing rider Fermin Aldeguer was declared unfit for the Dutch Grand Prix following Practice due to a fracture to his T7 vertebra.
Castrol Honda LCR rider Johann Zarco (fibula fracture recovery) was ruled out of the Dutch Grand Prix.