MotoGP Second Free Practice Results Misano San Marino Saturday September 12
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Marc Marquez | Ducati Lenovo | 300.0 | 01:31.040 | |
| 2 | Jorge Martin | Aprilia Racing | 300.8 | 01:31.047 | 0.007 / 0.007 |
| 3 | Marco Bezzecchi | Aprilia Racing | 303.3 | 01:31.079 | 0.039 / 0.032 |
| 4 | Franco Morbidelli | Pertamina Enduro VR46 | 295.8 | 01:31.278 | 0.238 / 0.199 |
| 5 | Alex Marquez | Gresini Racing | 300.0 | 01:31.300 | 0.260 / 0.022 |
| 6 | Raul Fernandez | Trackhouse Racing | 296.7 | 01:31.315 | 0.275 / 0.015 |
| 7 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 299.1 | 01:31.337 | 0.297 / 0.022 |
| 8 | Francesco Bagnaia | Ducati Lenovo | 301.6 | 01:31.359 | 0.319 / 0.022 |
| 9 | Fabio Quartararo | Monster Energy Yamaha | 294.2 | 01:31.470 | 0.430 / 0.111 |
| 10 | Pedro Acosta | Red Bull KTM | 300.8 | 01:31.694 | 0.654 / 0.224 |
| 11 | Fermin Aldeguer | Gresini Racing | 296.7 | 01:31.704 | 0.664 / 0.010 |
| 12 | Johann Zarco | Castrol Honda LCR | 298.3 | 01:31.816 | 0.776 / 0.112 |
| 13 | Joan Mir | Honda HRC Castrol | 300.0 | 01:31.920 | 0.880 / 0.104 |
| 14 | Pol Espargaro* | KTM Tech3 | 300.0 | 01:31.988 | 0.948 / 0.068 |
| 15 | Diogo Moreira | Pro Honda LCR | 300.0 | 01:32.012 | 0.972 / 0.024 |
| 16 | Enea Bastianini | KTM Tech3 | 297.5 | 01:32.046 | 1.006 / 0.034 |
| 17 | Brad Binder | Red Bull KTM | 299.1 | 01:32.050 | 1.010 / 0.004 |
| 18 | Luca Marini | Honda HRC Castrol | 302.5 | 01:32.073 | 1.033 / 0.023 |
| 19 | Toprak Razgatlıoğlu | Pramac Racing | 292.6 | 01:32.175 | 1.135 / 0.102 |
| 20 | Jack Miller | Pramac Racing | 293.4 | 01:32.473 | 1.433 / 0.298 |
| 21 | Alex Rins | Monster Energy Yamaha | 296.7 | 01:32.830 | 1.790 / 0.357 |
*Replaces Maverick Viñales for the San Marino Grand Prix
Note: Red Bull KTM Tech3 rider Maverick Viñales (left shoulder injury recovery) and SuperFile Trackhouse's Ai Ogura (left hand injury recovery) were ruled out of the San Marino Grand Prix.