MotoGP First Free Practice Results Mugello Italy Friday May 29
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 351.7 | 01:46.242 | |
| 2 | Jorge Martin | Aprilia Racing | 357.6 | 01:46.611 | 0.369 / 0.369 |
| 3 | Ai Ogura | Trackhouse Racing | 356.4 | 01:46.680 | 0.438 / 0.069 |
| 4 | Maverick Viñales | KTM Tech3 | 356.4 | 01:46.694 | 0.452 / 0.014 |
| 5 | Jack Miller | Pramac Racing | 351.7 | 01:46.744 | 0.502 / 0.050 |
| 6 | Pedro Acosta | Red Bull KTM | 360.0 | 01:46.794 | 0.552 / 0.050 |
| 7 | Fermin Aldeguer | Gresini Racing | 349.5 | 01:46.916 | 0.674 / 0.122 |
| 8 | Francesco Bagnaia | Ducati Lenovo | 355.2 | 01:46.971 | 0.729 / 0.055 |
| 9 | Raul Fernandez | Trackhouse Racing | 351.7 | 01:47.072 | 0.830 / 0.101 |
| 10 | Franco Morbidelli | Pertamina Enduro VR46 | 351.7 | 01:47.238 | 0.996 / 0.166 |
| 11 | Luca Marini | Honda HRC Castrol | 355.2 | 01:47.246 | 1.004 / 0.008 |
| 12 | Brad Binder | Red Bull KTM | 358.8 | 01:47.277 | 1.035 / 0.031 |
| 13 | Joan Mir | Honda HRC Castrol | 356.4 | 01:47.392 | 1.150 / 0.115 |
| 14 | Marco Bezzecchi | Aprilia Racing | 360.0 | 01:47.415 | 1.173 / 0.023 |
| 15 | Marc Marquez | Ducati Lenovo | 351.7 | 01:47.419 | 1.177 / 0.004 |
| 16 | Michele Pirro* | Gresini Racing | 347.2 | 01:47.422 | 1.180 / 0.003 |
| 17 | Enea Bastianini | KTM Tech3 | 362.4 | 01:47.448 | 1.206 / 0.026 |
| 18 | Diogo Moreira | Pro Honda LCR | 358.8 | 01:47.520 | 1.278 / 0.072 |
| 19 | Fabio Quartararo | Monster Energy Yamaha | 347.2 | 01:47.550 | 1.308 / 0.030 |
| 20 | Alex Rins | Monster Energy Yamaha | 354.0 | 01:47.743 | 1.501 / 0.193 |
| 21 | Toprak Razgatlıoğlu | Pramac Racing | 355.2 | 01:48.065 | 1.823 / 0.322 |
| 22 | Cal Crutchlow^ | Castrol Honda LCR | 349.5 | 01:49.913 | 3.671 / 1.848 |
*Replaced Alex Marquez for the Italian Grand Prix
^Replaced Johann Zarco for the Italian Grand Prix
Note: Gresini Racing rider Alex Marquez (collarbone injury recovery) and Castrol Honda LCR's Johann Zarco (fibula fracture recovery) were ruled out of the Italian Grand Prix.