| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Scott Redding | Octo Pramac Yakhnich | 286.9 | 02:09.500 | ||||
| 2 | Andrea Iannone | Ducati | 280.4 | 02:10.546 | 1.046 / 1.046 | |||
| 3 | Loris Baz | Avintia Racing | 266.5 | 02:10.955 | 1.455 / 0.409 | |||
| 4 | Marc Marquez | Repsol Honda | 277.8 | 02:11.083 | 1.583 / 0.128 | |||
| 5 | Eugene Laverty | Pull & Bear Aspar | 281.3 | 02:11.140 | 1.640 / 0.057 | |||
| 6 | Andrea Dovizioso | Ducati | 281.1 | 02:11.150 | 1.650 / 0.010 | |||
| 7 | Maverick Viñales | Suzuki Ecstar | 291.5 | 02:11.406 | 1.906 / 0.256 | |||
| 8 | Bradley Smith | Tech 3 | 270.3 | 02:11.536 | 2.036 / 0.130 | |||
| 9 | Cal Crutchlow | LCR Honda | 286.9 | 02:11.734 | 2.234 / 0.198 | |||
| 10 | Valentino Rossi | Movistar Yamaha | 279.3 | 02:11.787 | 2.287 / 0.053 | |||
| 11 | Alvaro Bautista | Aprilia Gresini | 263.7 | 02:12.445 | 2.945 / 0.658 | |||
| 12 | Pol Espargaro | Tech 3 | 262.3 | 02:12.699 | 3.199 / 0.254 | |||
| 13 | Hector Barbera | Avintia Racing | 273.6 | 02:12.748 | 3.248 / 0.049 | |||
| 14 | Danilo Petrucci | Octo Pramac Yakhnich | 270.0 | 02:12.763 | 3.263 / 0.015 | |||
| 15 | Stefan Bradl | Aprilia Gresini | 277.3 | 02:12.981 | 3.481 / 0.218 | |||
| 16 | Dani Pedrosa | Repsol Honda | 258.2 | 02:13.274 | 3.774 / 0.293 | |||
| 17 | Jorge Lorenzo | Movistar Yamaha | 266.7 | 02:13.683 | 4.183 / 0.409 | |||
| 18 | Aleix Espargaro | Suzuki Ecstar | 278.7 | 02:13.715 | 4.215 / 0.032 | |||
| 19 | Yonny Hernandez | Pull & Bear Aspar | 280.5 | 02:14.227 | 4.727 / 0.512 | |||
| 20 | Tito Rabat | EG 0,0 Marc VDS | 274.2 | 02:15.784 | 6.284 / 1.557 |
Note: Team Estrella Galicia 0,0 Marc VDS rider Jack Miller did not take part in the Czech Republic Grand Prix due to injuries sustained in Austria