| 1. | Jorge Lorenzo | Yamaha Factory | 01:48.076 | |
| 2. | Marc Marquez | Repsol Honda | 01:48.434 | |
| 3. | Dani Pedrosa | Repsol Honda | 01:48.928 | |
| 4. | Valentino Rossi | Yamaha Factory | 01:49.007 | |
| 5. | Stefan Bradl | LCR Honda | 01:49.553 | |
| 6. | Alvaro Bautista | Go&Fun Honda | 01:50.080 | |
| 7. | Randy de Puniet | Suzuki | 01:50.860 |
Note: Wednesday's test was attended by the Yamaha Factory Racing team, the Suzuki test team and all of the Honda teams. Only the Repsol Honda team and the Suzuki test team will continue testing at Motorland Aragon on Thursday.
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