| 1. | Aleix Espargaro | Forward Racing | 01:55.386 | 43 |
| 2. | Bradley Smith | Tech 3 | 01:55.639 | 53 |
| 3. | Pol Espargaro | Tech 3 | 01:56.072 | 62 |
| 4. | Alvaro Bautista | Go&Fun Honda | 01:56.081 | 52 |
| 5. | Stefan Bradl | LCR Honda | 01:56.081 | 49 |
| 6. | Andrea Iannone | Pramac Racing | 01:56.780 | 63 |
| 7. | Colin Edwards | Forward Racing | 01:56.961 | 34 |
| 8. | Yonny Hernandez | Pramac Racing | 01:57.318 | 63 |
| 9. | Nicky Hayden | Drive M7 Aspar | 01:57.574 | 48 |
| 10. | Scott Redding | Go&Fun Honda | 01:57.922 | 38 |
| 11. | Hiroshi Aoyama | Drive M7 Aspar | 01:58.151 | 55 |
| 12. | Hector Barbera | Avintia Racing | 01:58.503 | 34 |
| 13. | Karel Abraham | Cardion AB | 01:58.833 | 49 |
| 14. | Broc Parkes | Paul Bird | 01:59.121 | 30 |
| 15. | Mike di Meglio | Avintia Racing | 01:59.294 | 52 |
| 16. | Danilo Petrucci | IodaRacing | 01:59.358 | 57 |
| 17. | Michael Laverty | Paul Bird | 02:00.055 | 35 |
Note: Repsol Honda (Marc Marquez and Dani Pedrosa), Movistar Yamaha MotoGP (Jorge Lorenzo and Valentino Rossi) and Ducati Team (Andrea Dovizioso and Cal Crutchlow) were not taking part in the three-day test at the Losail International Circuit in Qatar.
