| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Jorge Lorenzo | Movistar Yamaha | 01:55.394 | 22 / 43 | |||
| 2 | Valentino Rossi | Movistar Yamaha | 01:55.765 | 0.371 | 0.371 | 57 / 60 | |
| 3 | Marc Marquez | Repsol Honda | 01:56.148 | 0.754 | 0.383 | 39 / 46 | |
| 4 | Cal Crutchlow | LCR Honda | 01:56.273 | 0.879 | 0.125 | 55 / 63 | |
| 5 | Maverick Viñales | Suzuki Ecstar | 01:56.524 | 1.130 | 0.251 | 29 / 37 | |
| 6 | Bradley Smith | Tech 3 | 01:56.619 | 1.225 | 0.095 | 46 / 47 | |
| 7 | Dani Pedrosa | Repsol Honda | 01:56.857 | 1.463 | 0.238 | 29 / 43 | |
| 8 | Pol Espargaro | Tech 3 | 01:56.952 | 1.558 | 0.095 | 22 / 43 | |
| 9 | Scott Redding | Octo Pramac Yakhnich | 01:57.077 | 1.683 | 0.125 | 44 / 47 | |
| 10 | Tito Rabat | EG 0,0 Marc VDS | 01:57.863 | 2.469 | 0.786 | 75 / 83 | |
| 11 | Danilo Petrucci | Octo Pramac Yakhnich | 01:58.421 | 3.027 | 0.558 | 9 / 18 | |
| 12 | Alex Lowes* | Tech 3 | 01:59.558 | 4.164 | 1.137 | 15 / 15 |
*Pata Yamaha WSBK rider
Note: Ducati Team, Avintia Racing, Aprilia Racing Team Gresini and Aspar Team MotoGP did not take part in the one day official post-race test in Brno