| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Maverick Viñales | Movistar Yamaha | 01:29.975 | 66 / 76 | |||
| 2 | Marc Marquez | Repsol Honda | 01:30.171 | 0.196 | 0.196 | 58 / 78 | |
| 3 | Andrea Dovizioso | Ducati | 01:30.443 | 0.468 | 0.272 | 33 / 61 | |
| 4 | Andrea Iannone | Suzuki Ecstar | 01:30.599 | 0.624 | 0.156 | 47 / 51 | |
| 5 | Dani Pedrosa | Repsol Honda | 01:30.686 | 0.711 | 0.087 | 38 / 41 | |
| 6 | Cal Crutchlow | LCR Honda | 01:30.709 | 0.734 | 0.023 | 45 / 63 | |
| 7 | Valentino Rossi | Movistar Yamaha | 01:30.709 | 0.734 | 0.000 | 45 / 69 | |
| 8 | Jorge Lorenzo | Ducati | 01:30.744 | 0.769 | 0.035 | 41 / 66 | |
| 9 | Aleix Espargaro | Aprilia Gresini | 01:30.885 | 0.910 | 0.141 | 45 / 61 | |
| 10 | Jonas Folger | Tech 3 | 01:30.948 | 0.973 | 0.063 | 62 / 63 | |
| 11 | Johann Zarco | Tech 3 | 01:31.015 | 1.040 | 0.067 | 55 / 61 | |
| 12 | Jack Miller | EG 0,0 Marc VDS | 01:31.069 | 1.094 | 0.054 | 40 / 59 | |
| 13 | Scott Redding | Octo Pramac Yakhnich | 01:31.118 | 1.143 | 0.049 | 42 / 48 | |
| 14 | Alvaro Bautista | Pull & Bear Aspar | 01:31.186 | 1.211 | 0.068 | 59 / 69 | |
| 15 | Hector Barbera | Avintia Racing | 01:31.244 | 1.269 | 0.058 | 39 / 59 | |
| 16 | Michele Pirro* | Octo Pramac Yakhnich | 01:31.816 | 1.841 | 0.572 | 37 / 40 | |
| 17 | Pol Espargaro | Red Bull KTM | 01:31.853 | 1.878 | 0.037 | 33 / 70 | |
| 18 | Tito Rabat | EG 0,0 Marc VDS | 01:31.918 | 1.943 | 0.065 | 36 / 61 | |
| 19 | Loris Baz | Avintia Racing | 01:32.161 | 2.186 | 0.243 | 36 / 52 | |
| 20 | Bradley Smith | Red Bull KTM | 01:32.538 | 2.563 | 0.377 | 34 / 59 | |
| 21 | Eugene Laverty^ | Aprilia | 01:32.568 | 2.593 | 0.030 | 38 / 57 | |
| 22 | Karel Abraham | Pull & Bear Aspar | 01:32.699 | 2.724 | 0.131 | 31 / 41 | |
| 23 | Takuya Tsuda^ | Suzuki | 01:33.305 | 3.330 | 0.606 | 68 / 77 | |
| 24 | Alex Rins | Suzuki Ecstar | 01:33.761 | 3.786 | 0.456 | 4 / 5 |
*Replaced Danilo Petrucci for the Valencia Test
^Test riders
Note: Aprilia Racing Team Gresini rider Sam Lowes did not take part in the Wednesday session following his crash on Tuesday
This week's two-day test marked the start of the 2017 MotoGP season