| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Danilo Petrucci | Octo Pramac Racing | 290.0 | 01:33.231 | ||||
| 2 | Maverick Viñales | Movistar Yamaha | 288.7 | 01:33.236 | 0.005 / 0.005 | |||
| 3 | Andrea Dovizioso | Ducati | 288.5 | 01:33.294 | 0.063 / 0.058 | |||
| 4 | Dani Pedrosa | Repsol Honda | 290.2 | 01:33.346 | 0.115 / 0.052 | |||
| 5 | Marc Marquez | Repsol Honda | 287.3 | 01:33.475 | 0.244 / 0.129 | |||
| 6 | Cal Crutchlow | LCR Honda | 287.6 | 01:33.545 | 0.314 / 0.070 | |||
| 7 | Michele Pirro* | Ducati | 288.9 | 01:33.591 | 0.360 / 0.046 | |||
| 8 | Jorge Lorenzo | Ducati | 287.8 | 01:33.614 | 0.383 / 0.023 | |||
| 9 | Andrea Iannone | Suzuki Ecstar | 284.7 | 01:33.740 | 0.509 / 0.126 | |||
| 10 | Tito Rabat | EG 0,0 Marc VDS | 289.1 | 01:33.820 | 0.589 / 0.080 | |||
| 11 | Aleix Espargaro | Aprilia Gresini | 285.7 | 01:33.832 | 0.601 / 0.012 | |||
| 12 | Johann Zarco | Tech 3 | 285.5 | 01:33.914 | 0.683 / 0.082 | |||
| 13 | Alvaro Bautista | Pull&Bear Aspar | 290.5 | 01:33.937 | 0.706 / 0.023 | |||
| 14 | Loris Baz | Reale Avintia Racing | 283.1 | 01:34.054 | 0.823 / 0.117 | |||
| 15 | Hector Barbera | Reale Avintia Racing | 293.7 | 01:34.137 | 0.906 / 0.083 | |||
| 16 | Scott Redding | Octo Pramac Racing | 284.5 | 01:34.266 | 1.035 / 0.129 | |||
| 17 | Jonas Folger | Tech 3 | 285.4 | 01:34.322 | 1.091 / 0.056 | |||
| 18 | Jack Miller | EG 0,0 Marc VDS | 286.7 | 01:34.411 | 1.180 / 0.089 | |||
| 19 | Pol Espargaro | Red Bull KTM | 288.3 | 01:34.499 | 1.268 / 0.088 | |||
| 20 | Karel Abraham | Pull&Bear Aspar | 287.8 | 01:34.678 | 1.447 / 0.179 | |||
| 21 | Bradley Smith | Red Bull KTM | 287.0 | 01:34.842 | 1.611 / 0.164 | |||
| 22 | Sam Lowes | Aprilia Gresini | 282.3 | 01:34.903 | 1.672 / 0.061 | |||
| 23 | Alex Rins | Suzuki Ecstar | 286.6 | 01:35.052 | 1.821 / 0.149 |
*Wild card entry
Note: Movistar Yamaha MotoGP rider Valentino Rossi did not take part in the San Marino Grand Prix after breaking his tibia and fibula in a training crash
Riders and teams trialled a new potential flag-to-flag procedure at the end of the session
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