| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Marc Marquez | Repsol Honda | 01:55.209 | 67 / 77 | |||
| 2 | Johann Zarco | Tech 3 | 01:55.342 | 0.133 | 0.133 | 69 / 71 | |
| 3 | Dani Pedrosa | Repsol Honda | 01:55.399 | 0.190 | 0.057 | 46 / 52 | |
| 4 | Andrea Dovizioso | Ducati | 01:55.517 | 0.308 | 0.118 | 21 / 40 | |
| 5 | Jorge Lorenzo | Ducati | 01:55.609 | 0.400 | 0.092 | 3 / 45 | |
| 6 | Cal Crutchlow | LCR Honda Castrol | 01:55.771 | 0.562 | 0.162 | 20 / 43 | |
| 7 | Valentino Rossi | Movistar Yamaha | 01:55.918 | 0.709 | 0.147 | 26 / 51 | |
| 8 | Danilo Petrucci | Alma Pramac Racing | 01:55.939 | 0.730 | 0.021 | 17 / 34 | |
| 9 | Alex Rins | Suzuki Ecstar | 01:55.986 | 0.777 | 0.047 | 41 / 62 | |
| 10 | Hafizh Syahrin | Tech 3 | 01:56.032 | 0.823 | 0.046 | 58 / 65 | |
| 11 | Andrea Iannone | Suzuki Ecstar | 01:56.111 | 0.902 | 0.079 | 6 / 26 | |
| 12 | Aleix Espargaro | Aprilia Gresini | 01:56.366 | 1.157 | 0.255 | 10 / 26 | |
| 13 | Bradley Smith | Red Bull KTM | 01:56.566 | 1.357 | 0.200 | 26 / 68 | |
| 14 | Takaaki Nakagami | LCR Honda Idemitsu | 01:56.622 | 1.413 | 0.056 | 4 / 18 | |
| 15 | Franco Morbidelli | EG 0,0 Marc VDS | 01:56.697 | 1.488 | 0.075 | 19 / 30 | |
| 16 | Scott Redding | Aprilia Gresini | 01:56.855 | 1.646 | 0.158 | 41 / 50 | |
| 17 | Jack Miller | Alma Pramac Racing | 01:56.885 | 1.676 | 0.030 | 3 / 13 | |
| 18 | Thomas Luthi | EG 0,0 Marc VDS | 01:57.034 | 1.825 | 0.149 | 36 / 37 | |
| 19 | Sylvain Guintoli* | Suzuki Ecstar | 01:57.843 | 2.634 | 0.809 | 15 / 37 | |
| 20 | Hiroshi Aoyama* | HRC Honda | 01:59.334 | 4.125 | 1.491 | 36 / 43 |
*Test rider
Note: Angel Nieto Team, Reale Avintia Racing, Movistar Yamaha MotoGP's Maverick Viñales and Red Bull KTM Factory Racing rider Pol Espargaro did not take part in the official post Grand Prix test in Brno