| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Maverick Viñales | Monster Energy Yamaha | 01:38.967 | 98 / 98 | |||
| 2 | Franco Morbidelli | Petronas Yamaha | 01:39.014 | 0.047 | 0.047 | 76 / 78 | |
| 3 | Marc Marquez | Repsol Honda | 01:39.257 | 0.290 | 0.243 | 61 / 80 | |
| 4 | Alex Rins | Suzuki Ecstar | 01:39.334 | 0.367 | 0.077 | 72 / 75 | |
| 5 | Fabio Quartararo | Petronas Yamaha | 01:39.428 | 0.461 | 0.094 | 55 / 56 | |
| 6 | Francesco Bagnaia | Pramac Racing | 01:39.466 | 0.499 | 0.038 | 54 / 54 | |
| 7 | Takaaki Nakagami | LCR Honda Idemitsu | 01:39.555 | 0.588 | 0.089 | 52 / 59 | |
| 8 | Joan Mir | Suzuki Ecstar | 01:39.670 | 0.703 | 0.115 | 64 / 67 | |
| 9 | Jack Miller | Pramac Racing | 01:39.745 | 0.778 | 0.075 | 59 / 60 | |
| 10 | Cal Crutchlow | LCR Honda Castrol | 01:39.903 | 0.936 | 0.158 | 34 / 73 | |
| 11 | Danilo Petrucci | Mission Winnow Ducati | 01:39.917 | 0.950 | 0.014 | 31 / 57 | |
| 12 | Andrea Dovizioso | Mission Winnow Ducati | 01:39.925 | 0.958 | 0.008 | 16 / 63 | |
| 13 | Tito Rabat | Reale Avintia Racing | 01:39.938 | 0.971 | 0.013 | 26 / 75 | |
| 14 | Valentino Rossi | Monster Energy Yamaha | 01:39.968 | 1.001 | 0.030 | 58 / 71 | |
| 15 | Johann Zarco | Red Bull KTM | 01:40.197 | 1.230 | 0.229 | 68 / 70 | |
| 16 | Pol Espargaro | Red Bull KTM | 01:40.201 | 1.234 | 0.004 | 5 / 57 | |
| 17 | Jorge Lorenzo | Repsol Honda | 01:40.357 | 1.390 | 0.156 | 7 / 65 | |
| 18 | Bradley Smith* | Aprilia Gresini | 01:40.564 | 1.597 | 0.207 | 87 / 88 | |
| 19 | Andrea Iannone | Aprilia Gresini | 01:40.606 | 1.639 | 0.042 | 75 / 76 | |
| 20 | Miguel Oliveira | KTM Tech 3 | 01:40.862 | 1.895 | 0.256 | 31 / 61 | |
| 21 | Karel Abraham | Reale Avintia Racing | 01:41.100 | 2.133 | 0.238 | 34 / 35 | |
| 22 | Hafizh Syahrin | KTM Tech 3 | 01:41.162 | 2.195 | 0.062 | 50 / 52 | |
| 23 | Sylvain Guintoli* | Suzuki Ecstar | 01:41.243 | 2.276 | 0.081 | 18 / 69 | |
| 24 | Stefan Bradl* | Honda | 01:41.429 | 2.462 | 0.186 | 75 / 84 | |
| 25 | Dani Pedrosa* | Red Bull KTM | 01:41.824 | 2.857 | 0.395 | 38 / 51 |
*Test rider (4)
Note: Aprilia Racing Team Gresini rider Aleix Espargaro did not take part in the one-day MotoGP test at the Circuit de Barcelona-Catalunya