| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Fabio Quartararo | Petronas Yamaha | 01:30.163 | 73 / 82 | |||
| 2 | Maverick Viñales | Monster Energy Yamaha | 01:30.327 | 0.164 | 0.164 | 58 / 66 | |
| 3 | Franco Morbidelli | Petronas Yamaha | 01:30.650 | 0.487 | 0.323 | 30 / 71 | |
| 4 | Andrea Dovizioso | Ducati | 01:30.665 | 0.502 | 0.015 | 48 / 58 | |
| 5 | Marc Marquez | Repsol Honda | 01:30.698 | 0.535 | 0.033 | 33 / 60 | |
| 6 | Joan Mir | Suzuki Ecstar | 01:30.811 | 0.648 | 0.113 | 61 / 63 | |
| 7 | Alex Rins | Suzuki Ecstar | 01:30.958 | 0.795 | 0.147 | 57 / 76 | |
| 8 | Pol Espargaro | Red Bull KTM | 01:30.974 | 0.811 | 0.016 | 29 / 58 | |
| 9 | Valentino Rossi | Monster Energy Yamaha | 01:31.012 | 0.849 | 0.038 | 57 / 73 | |
| 10 | Jack Miller | Pramac Racing | 01:31.130 | 0.967 | 0.118 | 20 / 43 | |
| 11 | Cal Crutchlow | LCR Honda Castrol | 01:31.183 | 1.020 | 0.053 | 63 / 69 | |
| 12 | Danilo Petrucci | Ducati | 01:31.433 | 1.270 | 0.250 | 9 / 10 | |
| 13 | Iker Lecuona | KTM Tech 3 | 01:31.645 | 1.482 | 0.212 | 55 / 57 | |
| 14 | Andrea Iannone | Aprilia Gresini | 01:31.674 | 1.511 | 0.029 | 28 / 54 | |
| 15 | Tito Rabat | Reale Avintia Racing | 01:31.775 | 1.612 | 0.101 | 50 / 53 | |
| 16 | Aleix Espargaro | Aprilia Gresini | 01:31.815 | 1.652 | 0.040 | 20 / 45 | |
| 17 | Dani Pedrosa*^ | KTM Tech 3 | 01:31.863 | 1.700 | 0.048 | 38 / 52 | |
| 18 | Michele Pirro*° | Pramac Racing | 01:32.016 | 1.853 | 0.153 | 23 / 38 | |
| 19 | Karel Abraham | Reale Avintia Racing | 01:32.034 | 1.871 | 0.018 | 44 / 47 | |
| 20 | Bradley Smith* | Aprilia Gresini | 01:32.090 | 1.927 | 0.056 | 46 / 50 | |
| 21 | Brad Binder | Red Bull KTM | 01:32.645 | 2.482 | 0.555 | 61 / 70 | |
| 22 | Stefan Bradl* | Repsol Honda | 01:32.833 | 2.670 | 0.188 | 25 / 25 | |
| 23 | Alex Marquez+ | LCR Honda | 01:32.873 | 2.710 | 0.040 | 52 / 53 |
*Test rider (4)
^Replaced Miguel Oliveira for the MotoGP test in Valencia
°Replaced Francesco Bagnaia for the MotoGP test in Valencia
+Tested Takaaki Nakagami's 2019-spec Honda RC213V
Note: Pramac Racing rider Francesco Bagnaia and LCR Honda Idemitsu's Takaaki Nakagami did not take part in the MotoGP test in Valencia
This two-day test marked the start of the 2020 MotoGP season