| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Maverick Viñales | Monster Energy Yamaha | 01:29.849 | 56 / 67 | |||
| 2 | Fabio Quartararo | Petronas Yamaha | 01:30.013 | 0.164 | 0.164 | 42 / 64 | |
| 3 | Franco Morbidelli | Petronas Yamaha | 01:30.114 | 0.265 | 0.101 | 50 / 52 | |
| 4 | Cal Crutchlow | LCR Honda Castrol | 01:30.316 | 0.467 | 0.202 | 59 / 73 | |
| 5 | Joan Mir | Suzuki Ecstar | 01:30.427 | 0.578 | 0.111 | 54 / 61 | |
| 6 | Alex Rins | Suzuki Ecstar | 01:30.503 | 0.654 | 0.076 | 54 / 57 | |
| 7 | Marc Marquez | Repsol Honda | 01:30.556 | 0.707 | 0.053 | 53 / 71 | |
| 8 | Pol Espargaro | Red Bull KTM | 01:30.685 | 0.836 | 0.129 | 12 / 46 | |
| 9 | Valentino Rossi | Monster Energy Yamaha | 01:30.781 | 0.932 | 0.096 | 55 / 68 | |
| 10 | Jack Miller | Pramac Racing | 01:30.854 | 1.005 | 0.073 | 57 / 59 | |
| 11 | Andrea Dovizioso | Ducati | 01:31.143 | 1.294 | 0.289 | 37 / 42 | |
| 12 | Tito Rabat | Reale Avintia Racing | 01:31.258 | 1.409 | 0.115 | 62 / 63 | |
| 13 | Michele Pirro* | Ducati | 01:31.424 | 1.575 | 0.166 | 31 / 40 | |
| 14 | Dani Pedrosa*^ | KTM Tech 3 | 01:31.470 | 1.621 | 0.046 | 28 / 48 | |
| 15 | Iker Lecuona | KTM Tech 3 | 01:31.515 | 1.666 | 0.045 | 10 / 57 | |
| 16 | Aleix Espargaro | Aprilia Gresini | 01:31.526 | 1.677 | 0.011 | 22 / 43 | |
| 17 | Karel Abraham | Reale Avintia Racing | 01:31.597 | 1.748 | 0.071 | 53 / 59 | |
| 18 | Stefan Bradl* | Repsol Honda | 01:31.657 | 1.808 | 0.060 | 29 / 54 | |
| 19 | Andrea Iannone | Aprilia Gresini | 01:31.800 | 1.951 | 0.143 | 36 / 38 | |
| 20 | Alex Marquez+ | LCR Honda | 01:32.235 | 2.386 | 0.435 | 52 / 79 | |
| 21 | Bradley Smith* | Aprilia Gresini | 01:32.249 | 2.400 | 0.014 | 26 / 32 | |
| 22 | Brad Binder | Red Bull KTM | 01:32.367 | 2.518 | 0.118 | 61 / 66 |
*Test rider (4)
^Replaced Miguel Oliveira for the MotoGP test in Valencia
+Tested Takaaki Nakagami's 2019-spec Honda RC213V
Note: Ducati Team rider Danilo Petrucci did not take part in Day 2 of testing in Valencia
Pramac Racing rider Francesco Bagnaia and LCR Honda Idemitsu's Takaaki Nakagami did not take part in the MotoGP test in Valencia
This two-day test marked the start of the 2020 MotoGP season