| P | Rider | Team | Lap time | Lead. Gap | Prev. Gap | Laps | |
|---|---|---|---|---|---|---|---|
| 1 | Fabio Quartararo | Petronas Yamaha | 01:54.038 | 52 / 65 | |||
| 2 | Alex Rins | Suzuki Ecstar | 01:54.200 | 0.162 | 0.162 | 52 / 53 | |
| 3 | Maverick Viñales | Monster Energy Yamaha | 01:54.264 | 0.226 | 0.064 | 53 / 68 | |
| 4 | Franco Morbidelli | Petronas Yamaha | 01:54.481 | 0.443 | 0.217 | 55 / 70 | |
| 5 | Francesco Bagnaia | Pramac Racing | 01:54.520 | 0.482 | 0.039 | 37 / 52 | |
| 6 | Johann Zarco | Reale Avintia Racing | 01:54.565 | 0.527 | 0.045 | 55 / 56 | |
| 7 | Joan Mir | Suzuki Ecstar | 01:54.612 | 0.574 | 0.047 | 23 / 52 | |
| 8 | Andrea Dovizioso | Ducati | 01:54.662 | 0.624 | 0.050 | 36 / 60 | |
| 9 | Valentino Rossi | Monster Energy Yamaha | 01:54.740 | 0.702 | 0.078 | 22 / 52 | |
| 10 | Takaaki Nakagami | LCR Honda Idemitsu | 01:54.759 | 0.721 | 0.019 | 30 / 43 | |
| 11 | Aleix Espargaro | Aprilia Gresini | 01:54.762 | 0.724 | 0.003 | 26 / 33 | |
| 12 | Danilo Petrucci | Ducati | 01:54.852 | 0.814 | 0.090 | 23 / 49 | |
| 13 | Pol Espargaro | Red Bull KTM | 01:54.890 | 0.852 | 0.038 | 25 / 54 | |
| 14 | Marc Marquez | Repsol Honda | 01:55.093 | 1.055 | 0.203 | 26 / 46 | |
| 15 | Jack Miller | Pramac Racing | 01:55.194 | 1.156 | 0.101 | 11 / 42 | |
| 16 | Tito Rabat | Reale Avintia Racing | 01:55.352 | 1.314 | 0.158 | 53 / 54 | |
| 17 | Miguel Oliveira | KTM Tech 3 | 01:55.600 | 1.562 | 0.248 | 20 / 53 | |
| 18 | Iker Lecuona | KTM Tech 3 | 01:55.644 | 1.606 | 0.044 | 45 / 53 | |
| 19 | Alex Marquez | Repsol Honda | 01:55.725 | 1.687 | 0.081 | 22 / 48 | |
| 20 | Brad Binder | Red Bull KTM | 01:55.930 | 1.892 | 0.205 | 19 / 57 | |
| 21 | Cal Crutchlow | LCR Honda Castrol | 01:56.178 | 2.140 | 0.248 | 26 / 27 | |
| 22 | Bradley Smith* | Aprilia Gresini | 01:56.407 | 2.369 | 0.229 | 49 / 52 |
*Test rider (1)
Note: Aprilia Racing Team Gresini's suspended rider Andrea Iannone did not take part in the MotoGP test in Qatar