| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Fabio Quartararo | Petronas Yamaha | 307.6 | 01:56.502 | ||||
| 2 | Franco Morbidelli | Petronas Yamaha | 306.8 | 01:56.509 | 0.007 / 0.007 | |||
| 3 | Miguel Oliveira | KTM Tech 3 | 311.2 | 01:56.550 | 0.048 / 0.041 | |||
| 4 | Johann Zarco | Hublot Reale Avintia | 313.0 | 01:56.583 | 0.081 / 0.033 | |||
| 5 | Maverick Viñales | Monster Energy Yamaha | 307.6 | 01:56.668 | 0.166 / 0.085 | |||
| 6 | Joan Mir | Suzuki Ecstar | 308.5 | 01:56.876 | 0.374 / 0.208 | |||
| 7 | Aleix Espargaro | Aprilia Gresini | 312.1 | 01:57.041 | 0.539 / 0.165 | |||
| 8 | Pol Espargaro | Red Bull KTM | 313.0 | 01:57.059 | 0.557 / 0.018 | |||
| 9 | Takaaki Nakagami | LCR Honda Idemitsu | 309.4 | 01:57.073 | 0.571 / 0.014 | |||
| 10 | Jack Miller | Pramac Racing | 312.1 | 01:57.109 | 0.607 / 0.036 | |||
| 11 | Brad Binder | Red Bull KTM | 308.5 | 01:57.279 | 0.777 / 0.170 | |||
| 12 | Valentino Rossi | Monster Energy Yamaha | 307.6 | 01:57.290 | 0.788 / 0.011 | |||
| 13 | Alex Rins | Suzuki Ecstar | 309.4 | 01:57.354 | 0.852 / 0.064 | |||
| 14 | Danilo Petrucci | Ducati | 312.1 | 01:57.620 | 1.118 / 0.266 | |||
| 15 | Andrea Dovizioso | Ducati | 313.0 | 01:57.635 | 1.133 / 0.015 | |||
| 16 | Alex Marquez | Repsol Honda | 311.2 | 01:57.688 | 1.186 / 0.053 | |||
| 17 | Tito Rabat | Hublot Reale Avintia | 305.9 | 01:57.777 | 1.275 / 0.089 | |||
| 18 | Cal Crutchlow | LCR Honda Castrol | 312.1 | 01:57.865 | 1.363 / 0.088 | |||
| 19 | Bradley Smith | Aprilia Gresini | 309.4 | 01:58.161 | 1.659 / 0.296 | |||
| 20 | Iker Lecuona | KTM Tech 3 | 307.6 | 01:58.378 | 1.876 / 0.217 | |||
| 21 | Stefan Bradl* | Repsol Honda | 311.2 | 01:58.424 | 1.922 / 0.046 |
*Replaced Marc Marquez for the Czech Republic Grand Prix
Notes: Pramac Racing rider Francesco Bagnaia was ruled out of the Czech Republic Grand Prix after sustaining a right knee injury in FP1
Repsol Honda rider Marc Marquez was ruled out of the Czech Republic Grand Prix after undergoing a second surgery on a fractured humerus
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