MotoGP Second Free Practice Results Assen Netherlands Saturday June 28
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Fabio Quartararo | Monster Energy Yamaha | 300.0 | 01:31.876 | |
| 2 | Francesco Bagnaia | Ducati Lenovo | 309.0 | 01:32.050 | 0.174 / 0.174 |
| 3 | Pedro Acosta | Red Bull KTM | 306.0 | 01:32.070 | 0.194 / 0.020 |
| 4 | Marco Bezzecchi | Aprilia Racing | 309.8 | 01:32.136 | 0.260 / 0.066 |
| 5 | Marc Marquez | Ducati Lenovo | 307.5 | 01:32.187 | 0.311 / 0.051 |
| 6 | Maverick Viñales | KTM Tech3 | 304.4 | 01:32.364 | 0.488 / 0.177 |
| 7 | Franco Morbidelli | Pertamina Enduro VR46 | 308.3 | 01:32.364 | 0.488 / 0.000 |
| 8 | Fermin Aldeguer | Gresini Racing | 309.0 | 01:32.374 | 0.498 / 0.010 |
| 9 | Alex Marquez | Gresini Racing | 308.3 | 01:32.384 | 0.508 / 0.010 |
| 10 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 309.0 | 01:32.402 | 0.526 / 0.018 |
| 11 | Miguel Oliveira | Pramac Racing | 302.2 | 01:32.430 | 0.554 / 0.028 |
| 12 | Raul Fernandez | Trackhouse Racing | 303.7 | 01:32.507 | 0.631 / 0.077 |
| 13 | Alex Rins | Monster Energy Yamaha | 305.2 | 01:32.530 | 0.654 / 0.023 |
| 14 | Ai Ogura | Trackhouse Racing | 301.4 | 01:32.596 | 0.720 / 0.066 |
| 15 | Enea Bastianini | KTM Tech3 | 306.0 | 01:32.633 | 0.757 / 0.037 |
| 16 | Jack Miller | Pramac Racing | 302.9 | 01:32.666 | 0.790 / 0.033 |
| 17 | Joan Mir | Honda HRC Castrol | 302.2 | 01:32.763 | 0.887 / 0.097 |
| 18 | Johann Zarco | Castrol Honda LCR | 302.2 | 01:32.852 | 0.976 / 0.089 |
| 19 | Brad Binder | Red Bull KTM | 309.8 | 01:32.873 | 0.997 / 0.021 |
| 20 | Aleix Espargaro* | Honda HRC Castrol | 309.0 | 01:33.100 | 1.224 / 0.227 |
| 21 | Lorenzo Savadori^ | Aprilia Racing | 303.7 | 01:33.248 | 1.372 / 0.148 |
| 22 | Somkiat Chantra | Idemitsu Honda LCR | 306.0 | 01:34.157 | 2.281 / 0.909 |
*Replaced Luca Marini for the Dutch Grand Prix
^Replaced Jorge Martin for the Dutch Grand Prix