MotoGP Second Free Practice Results Mugello Italy Saturday June 21
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Maverick Viñales | KTM Tech3 | 352.9 | 01:45.742 | |
| 2 | Marc Marquez | Ducati Lenovo | 352.9 | 01:45.758 | 0.016 / 0.016 |
| 3 | Fabio Quartararo | Monster Energy Yamaha | 349.5 | 01:45.846 | 0.104 / 0.088 |
| 4 | Alex Marquez | Gresini Racing | 352.9 | 01:45.952 | 0.210 / 0.106 |
| 5 | Pedro Acosta | Red Bull KTM | 360.0 | 01:45.974 | 0.232 / 0.022 |
| 6 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 355.2 | 01:46.021 | 0.279 / 0.047 |
| 7 | Marco Bezzecchi | Aprilia Racing | 360.0 | 01:46.060 | 0.318 / 0.039 |
| 8 | Franco Morbidelli | Pertamina Enduro VR46 | 360.0 | 01:46.062 | 0.320 / 0.002 |
| 9 | Fermin Aldeguer | Gresini Racing | 352.9 | 01:46.090 | 0.348 / 0.028 |
| 10 | Francesco Bagnaia | Ducati Lenovo | 355.2 | 01:46.190 | 0.448 / 0.100 |
| 11 | Enea Bastianini | KTM Tech3 | 361.2 | 01:46.387 | 0.645 / 0.197 |
| 12 | Joan Mir | Honda HRC Castrol | 350.6 | 01:46.454 | 0.712 / 0.067 |
| 13 | Takaaki Nakagami* | Honda HRC Castrol | 350.6 | 01:46.468 | 0.726 / 0.014 |
| 14 | Brad Binder | Red Bull KTM | 358.8 | 01:46.526 | 0.784 / 0.058 |
| 15 | Ai Ogura | Trackhouse Racing | 354.0 | 01:46.572 | 0.830 / 0.046 |
| 16 | Raul Fernandez | Trackhouse Racing | 348.3 | 01:46.644 | 0.902 / 0.072 |
| 17 | Miguel Oliveira | Pramac Racing | 354.0 | 01:46.649 | 0.907 / 0.005 |
| 18 | Johann Zarco | Castrol Honda LCR | 347.2 | 01:46.675 | 0.933 / 0.026 |
| 19 | Alex Rins | Monster Energy Yamaha | 352.9 | 01:46.689 | 0.947 / 0.014 |
| 20 | Jack Miller | Pramac Racing | 354.0 | 01:46.909 | 1.167 / 0.220 |
| 21 | Lorenzo Savadori^ | Aprilia Racing | 355.2 | 01:47.324 | 1.582 / 0.415 |
| 22 | Somkiat Chantra | Idemitsu Honda LCR | 352.9 | 01:48.072 | 2.330 / 0.748 |
*Replaced Luca Marini for the Italian Grand Prix
^Replaced Jorge Martin for the Italian Grand Prix