| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Jorge Lorenzo | Movistar Yamaha | 308.4 | 01:56.275 | ||||
| 2 | Andrea Iannone | Ducati | 311.5 | 01:56.277 | 0.002 / 0.002 | |||
| 3 | Marc Marquez | Repsol Honda | 308.1 | 01:56.399 | 0.124 / 0.122 | |||
| 4 | Valentino Rossi | Movistar Yamaha | 308.3 | 01:56.628 | 0.353 / 0.229 | |||
| 5 | Maverick Viñales | Suzuki Ecstar | 308.3 | 01:56.769 | 0.494 / 0.141 | |||
| 6 | Andrea Dovizioso | Ducati | 310.9 | 01:56.905 | 0.630 / 0.136 | |||
| 7 | Dani Pedrosa | Repsol Honda | 309.7 | 01:56.939 | 0.664 / 0.034 | |||
| 8 | Hector Barbera | Avintia Racing | 303.8 | 01:57.083 | 0.808 / 0.144 | |||
| 9 | Aleix Espargaro | Suzuki Ecstar | 303.2 | 01:57.163 | 0.888 / 0.080 | |||
| 10 | Cal Crutchlow | LCR Honda | 307.6 | 01:57.165 | 0.890 / 0.002 | |||
| 11 | Bradley Smith | Tech 3 | 304.1 | 01:57.447 | 1.172 / 0.282 | |||
| 12 | Pol Espargaro | Tech 3 | 307.0 | 01:57.473 | 1.198 / 0.026 | |||
| 13 | Scott Redding | Octo Pramac Yakhnich | 306.9 | 01:57.518 | 1.243 / 0.045 | |||
| 14 | Danilo Petrucci | Octo Pramac Yakhnich | 307.0 | 01:57.613 | 1.338 / 0.095 | |||
| 15 | Stefan Bradl | Aprilia Gresini | 307.7 | 01:57.941 | 1.666 / 0.328 | |||
| 16 | Alvaro Bautista | Aprilia Gresini | 305.6 | 01:58.119 | 1.844 / 0.178 | |||
| 17 | Eugene Laverty | Pull & Bear Aspar | 307.4 | 01:58.146 | 1.871 / 0.027 | |||
| 18 | Tito Rabat | EG 0,0 Marc VDS | 304.3 | 01:58.241 | 1.966 / 0.095 | |||
| 19 | Loris Baz | Avintia Racing | 302.1 | 01:58.426 | 2.151 / 0.185 | |||
| 20 | Yonny Hernandez | Pull & Bear Aspar | 304.9 | 01:58.604 | 2.329 / 0.178 |
Note: Team Estrella Galicia 0,0 Marc VDS rider Jack Miller did not take part in the Czech Republic Grand Prix due to injuries sustained in Austria