| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Jorge Lorenzo | Movistar Yamaha | 310.4 | 01:54.791 | ||||
| 2 | Andrea Iannone | Ducati | 312.4 | 01:55.535 | 0.744 / 0.744 | |||
| 3 | Marc Marquez | Repsol Honda | 307.8 | 01:55.562 | 0.771 / 0.027 | |||
| 4 | Maverick Viñales | Suzuki Ecstar | 309.0 | 01:55.604 | 0.813 / 0.042 | |||
| 5 | Valentino Rossi | Movistar Yamaha | 309.4 | 01:55.708 | 0.917 / 0.104 | |||
| 6 | Aleix Espargaro | Suzuki Ecstar | 306.0 | 01:55.793 | 1.002 / 0.085 | |||
| 7 | Andrea Dovizioso | Ducati | 312.4 | 01:56.051 | 1.260 / 0.258 | |||
| 8 | Pol Espargaro | Tech 3 | 309.1 | 01:56.143 | 1.352 / 0.092 | |||
| 9 | Cal Crutchlow | LCR Honda | 307.5 | 01:56.161 | 1.370 / 0.018 | |||
| 10 | Scott Redding | Octo Pramac Yakhnich | 304.6 | 01:56.332 | 1.541 / 0.171 | |||
| 11 | Dani Pedrosa | Repsol Honda | 311.0 | 01:56.405 | 1.614 / 0.073 | |||
| 12 | Danilo Petrucci | Octo Pramac Yakhnich | 309.5 | 01:56.551 | 1.760 / 0.146 | |||
| 13 | Bradley Smith | Tech 3 | 306.9 | 01:56.656 | 1.865 / 0.105 | |||
| 14 | Alvaro Bautista | Aprilia Gresini | 306.9 | 01:56.845 | 2.054 / 0.189 | |||
| 15 | Stefan Bradl | Aprilia Gresini | 308.3 | 01:57.015 | 2.224 / 0.170 | |||
| 16 | Eugene Laverty | Pull & Bear Aspar | 310.3 | 01:57.202 | 2.411 / 0.187 | |||
| 17 | Yonny Hernandez | Pull & Bear Aspar | 306.6 | 01:57.443 | 2.652 / 0.241 | |||
| 18 | Hector Barbera | Avintia Racing | 308.5 | 01:57.526 | 2.735 / 0.083 | |||
| 19 | Tito Rabat | EG 0,0 Marc VDS | 307.4 | 01:57.815 | 3.024 / 0.289 | |||
| 20 | Loris Baz | Avintia Racing | 303.1 | 01:58.144 | 3.353 / 0.329 |
Note: Team Estrella Galicia 0,0 Marc VDS rider Jack Miller did not take part in the Czech Republic Grand Prix due to injuries sustained in Austria