| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Andrea Iannone | Ducati | 311.3 | 01:56.189 | ||||
| 2 | Valentino Rossi | Movistar Yamaha | 305.6 | 01:56.454 | 0.265 / 0.265 | |||
| 3 | Jorge Lorenzo | Movistar Yamaha | 307.6 | 01:56.673 | 0.484 / 0.219 | |||
| 4 | Hector Barbera | Avintia Racing | 308.3 | 01:56.964 | 0.775 / 0.291 | |||
| 5 | Andrea Dovizioso | Ducati | 312.2 | 01:57.037 | 0.848 / 0.073 | |||
| 6 | Marc Marquez | Repsol Honda | 306.2 | 01:57.085 | 0.896 / 0.048 | |||
| 7 | Maverick Viñales | Suzuki Ecstar | 307.2 | 01:57.176 | 0.987 / 0.091 | |||
| 8 | Scott Redding | Octo Pramac Yakhnich | 306.2 | 01:57.504 | 1.315 / 0.328 | |||
| 9 | Dani Pedrosa | Repsol Honda | 309.2 | 01:57.524 | 1.335 / 0.020 | |||
| 10 | Aleix Espargaro | Suzuki Ecstar | 303.8 | 01:57.538 | 1.349 / 0.014 | |||
| 11 | Bradley Smith | Tech 3 | 306.2 | 01:57.599 | 1.410 / 0.061 | |||
| 12 | Cal Crutchlow | LCR Honda | 307.2 | 01:57.621 | 1.432 / 0.022 | |||
| 13 | Pol Espargaro | Tech 3 | 305.3 | 01:58.086 | 1.897 / 0.465 | |||
| 14 | Danilo Petrucci | Octo Pramac Yakhnich | 305.8 | 01:58.321 | 2.132 / 0.235 | |||
| 15 | Stefan Bradl | Aprilia Gresini | 304.1 | 01:58.481 | 2.292 / 0.160 | |||
| 16 | Yonny Hernandez | Pull & Bear Aspar | 304.9 | 01:58.660 | 2.471 / 0.179 | |||
| 17 | Alvaro Bautista | Aprilia Gresini | 300.1 | 01:58.685 | 2.496 / 0.025 | |||
| 18 | Tito Rabat | EG 0,0 Marc VDS | 301.7 | 01:58.834 | 2.645 / 0.149 | |||
| 19 | Loris Baz | Avintia Racing | 300.1 | 01:59.116 | 2.927 / 0.282 | |||
| 20 | Eugene Laverty | Pull & Bear Aspar | 306.5 | 01:59.352 | 3.163 / 0.236 |
Note: Team Estrella Galicia 0,0 Marc VDS rider Jack Miller did not take part in the Czech Republic Grand Prix due to injuries sustained in Austria