| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Marc Marquez | Repsol Honda | 307.8 | 01:55.840 | ||||
| 2 | Andrea Iannone | Ducati | 311.0 | 01:55.944 | 0.104 / 0.104 | |||
| 3 | Jorge Lorenzo | Movistar Yamaha | 307.2 | 01:55.977 | 0.137 / 0.033 | |||
| 4 | Hector Barbera | Avintia Racing | 307.7 | 01:56.124 | 0.284 / 0.147 | |||
| 5 | Valentino Rossi | Movistar Yamaha | 307.7 | 01:56.174 | 0.334 / 0.050 | |||
| 6 | Andrea Dovizioso | Ducati | 312.3 | 01:56.235 | 0.395 / 0.061 | |||
| 7 | Aleix Espargaro | Suzuki Ecstar | 305.9 | 01:56.441 | 0.601 / 0.206 | |||
| 8 | Bradley Smith | Tech 3 | 308.0 | 01:56.494 | 0.654 / 0.053 | |||
| 9 | Maverick Viñales | Suzuki Ecstar | 306.4 | 01:56.615 | 0.775 / 0.121 | |||
| 10 | Dani Pedrosa | Repsol Honda | 309.6 | 01:56.662 | 0.822 / 0.047 | |||
| 11 | Cal Crutchlow | LCR Honda | 306.6 | 01:56.837 | 0.997 / 0.175 | |||
| 12 | Loris Baz | Avintia Racing | 304.9 | 01:56.957 | 1.117 / 0.120 | |||
| 13 | Danilo Petrucci | Octo Pramac Yakhnich | 306.2 | 01:57.052 | 1.212 / 0.095 | |||
| 14 | Scott Redding | Octo Pramac Yakhnich | 308.0 | 01:57.090 | 1.250 / 0.038 | |||
| 15 | Pol Espargaro | Tech 3 | 306.1 | 01:57.335 | 1.495 / 0.245 | |||
| 16 | Eugene Laverty | Pull & Bear Aspar | 308.3 | 01:57.336 | 1.496 / 0.001 | |||
| 17 | Stefan Bradl | Aprilia Gresini | 304.3 | 01:57.471 | 1.631 / 0.135 | |||
| 18 | Alvaro Bautista | Aprilia Gresini | 303.4 | 01:57.569 | 1.729 / 0.098 | |||
| 19 | Yonny Hernandez | Pull & Bear Aspar | 306.9 | 01:57.724 | 1.884 / 0.155 | |||
| 20 | Tito Rabat | EG 0,0 Marc VDS | 305.5 | 01:58.054 | 2.214 / 0.330 |
Note: Team Estrella Galicia 0,0 Marc VDS rider Jack Miller did not take part in the Czech Republic Grand Prix due to injuries sustained in Austria