| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Maverick Viñales | Monster Energy Yamaha | 327.3 | 01:59.111 | ||||
| 2 | Cal Crutchlow | LCR Honda Castrol | 327.3 | 01:59.400 | 0.289 / 0.289 | |||
| 3 | Joan Mir | Suzuki Ecstar | 325.4 | 01:59.514 | 0.403 / 0.114 | |||
| 4 | Francesco Bagnaia | Pramac Racing | 331.3 | 01:59.711 | 0.600 / 0.197 | |||
| 5 | Valentino Rossi | Monster Energy Yamaha | 324.4 | 01:59.975 | 0.864 / 0.264 | |||
| 6 | Mika Kallio* | Red Bull KTM | 326.3 | 02:00.014 | 0.903 / 0.039 | |||
| 7 | Takaaki Nakagami | LCR Honda Idemitsu | 325.4 | 02:00.200 | 1.089 / 0.186 | |||
| 8 | Alex Rins | Suzuki Ecstar | 329.3 | 02:00.284 | 1.173 / 0.084 | |||
| 9 | Bradley Smith^ | Aprilia Gresini | 321.5 | 02:00.483 | 1.372 / 0.199 | |||
| 10 | Franco Morbidelli | Petronas Yamaha | 321.5 | 02:00.594 | 1.483 / 0.111 | |||
| 11 | Jorge Lorenzo | Repsol Honda | 327.3 | 02:00.654 | 1.543 / 0.060 | |||
| 12 | Miguel Oliveira | KTM Tech 3 | 321.5 | 02:00.661 | 1.550 / 0.007 | |||
| 13 | Danilo Petrucci | Ducati | 328.3 | 02:01.154 | 2.043 / 0.493 | |||
| 14 | Jack Miller | Pramac Racing | 332.3 | 02:01.181 | 2.070 / 0.027 | |||
| 15 | Hafizh Syahrin | KTM Tech 3 | 322.5 | 02:02.593 | 3.482 / 1.412 | |||
| 16 | Andrea Dovizioso | Ducati | 327.3 | 02:03.191 | 4.080 / 0.598 | |||
| 17 | Fabio Quartararo | Petronas Yamaha | 319.6 | 02:03.421 | 4.310 / 0.230 | |||
| 18 | Andrea Iannone | Aprilia Gresini | 315.9 | 02:08.271 | 9.160 / 4.850 | |||
| 19 | Karel Abraham | Reale Avintia Racing | no time | |||||
| 20 | Aleix Espargaro | Aprilia Gresini | no time | |||||
| 21 | Tito Rabat | Reale Avintia Racing | no time | |||||
| 22 | Marc Marquez | Repsol Honda | no time |
*Replaced Johann Zarco for the rest of the 2019 season
^Wild card entry
Note: Red Bull KTM Factory Racing rider Pol Espargaro was declared unfit for the Spanish Grand Prix after fracturing his left wrist in FP4