| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Fabio Quartararo | Petronas Yamaha | 304.2 | 01:56.959 | ||||
| 2 | Pol Espargaro | Red Bull KTM | 313.0 | 01:56.967 | 0.008 / 0.008 | |||
| 3 | Franco Morbidelli | Petronas Yamaha | 305.0 | 01:57.437 | 0.478 / 0.470 | |||
| 4 | Alex Rins | Suzuki Ecstar | 307.6 | 01:57.547 | 0.588 / 0.110 | |||
| 5 | Valentino Rossi | Monster Energy Yamaha | 309.4 | 01:57.751 | 0.792 / 0.204 | |||
| 6 | Andrea Dovizioso | Ducati | 314.8 | 01:57.844 | 0.885 / 0.093 | |||
| 7 | Joan Mir | Suzuki Ecstar | 308.5 | 01:57.919 | 0.960 / 0.075 | |||
| 8 | Aleix Espargaro | Aprilia Gresini | 310.3 | 01:57.979 | 1.020 / 0.060 | |||
| 9 | Brad Binder | Red Bull KTM | 312.1 | 01:58.002 | 1.043 / 0.023 | |||
| 10 | Takaaki Nakagami | LCR Honda Idemitsu | 308.5 | 01:58.032 | 1.073 / 0.030 | |||
| 11 | Miguel Oliveira | KTM Tech 3 | 305.9 | 01:58.085 | 1.126 / 0.053 | |||
| 12 | Johann Zarco | Hublot Reale Avintia | 310.3 | 01:58.106 | 1.147 / 0.021 | |||
| 13 | Maverick Viñales | Monster Energy Yamaha | 306.8 | 01:58.125 | 1.166 / 0.019 | |||
| 14 | Jack Miller | Pramac Racing | 315.7 | 01:58.231 | 1.272 / 0.106 | |||
| 15 | Alex Marquez | Repsol Honda | 309.4 | 01:58.366 | 1.407 / 0.135 | |||
| 16 | Danilo Petrucci | Ducati | 311.2 | 01:58.370 | 1.411 / 0.004 | |||
| 17 | Tito Rabat | Hublot Reale Avintia | 308.5 | 01:58.374 | 1.415 / 0.004 | |||
| 18 | Iker Lecuona | KTM Tech 3 | 305.0 | 01:58.433 | 1.474 / 0.059 | |||
| 19 | Cal Crutchlow | LCR Honda Castrol | 310.3 | 01:58.513 | 1.554 / 0.080 | |||
| 20 | Bradley Smith | Aprilia Gresini | 306.8 | 01:58.536 | 1.577 / 0.023 | |||
| 21 | Stefan Bradl* | Repsol Honda | 311.2 | 01:59.448 | 2.489 / 0.912 |
*Replaced Marc Marquez for the Czech Republic Grand Prix
Note: Pramac Racing rider Francesco Bagnaia was ruled out of the Czech Republic Grand Prix after sustaining a right knee injury in FP1
Repsol Honda rider Marc Marquez was ruled out of the Czech Republic Grand Prix after undergoing a second surgery on a fractured humerus
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