| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. | |||
|---|---|---|---|---|---|---|---|---|
| 1 | Franco Morbidelli | Petronas Yamaha | 307.6 | 01:56.037 | ||||
| 2 | Johann Zarco | Hublot Reale Avintia | 313.0 | 01:56.053 | 0.016 / 0.016 | |||
| 3 | Fabio Quartararo | Petronas Yamaha | 307.6 | 01:56.134 | 0.097 / 0.081 | |||
| 4 | Pol Espargaro | Red Bull KTM | 313.9 | 01:56.140 | 0.103 / 0.006 | |||
| 5 | Valentino Rossi | Monster Energy Yamaha | 306.8 | 01:56.150 | 0.113 / 0.010 | |||
| 6 | Aleix Espargaro | Aprilia Gresini | 311.2 | 01:56.183 | 0.146 / 0.033 | |||
| 7 | Joan Mir | Suzuki Ecstar | 310.3 | 01:56.261 | 0.224 / 0.078 | |||
| 8 | Cal Crutchlow | LCR Honda Castrol | 313.0 | 01:56.263 | 0.226 / 0.002 | |||
| 9 | Maverick Viñales | Monster Energy Yamaha | 308.5 | 01:56.282 | 0.245 / 0.019 | |||
| 10 | Danilo Petrucci | Ducati | 313.0 | 01:56.294 | 0.257 / 0.012 | |||
| 11 | Alex Rins | Suzuki Ecstar | 312.1 | 01:56.355 | 0.318 / 0.061 | |||
| 12 | Takaaki Nakagami | LCR Honda Idemitsu | 310.3 | 01:56.457 | 0.420 / 0.102 | |||
| 13 | Andrea Dovizioso | Ducati | 316.7 | 01:56.521 | 0.484 / 0.064 | |||
| 14 | Jack Miller | Pramac Racing | 313.9 | 01:56.590 | 0.553 / 0.069 | |||
| 15 | Brad Binder | Red Bull KTM | 310.3 | 01:56.730 | 0.693 / 0.140 | |||
| 16 | Tito Rabat | Hublot Reale Avintia | 308.5 | 01:57.031 | 0.994 / 0.301 | |||
| 17 | Bradley Smith | Aprilia Gresini | 309.4 | 01:57.052 | 1.015 / 0.021 | |||
| 18 | Miguel Oliveira | KTM Tech 3 | 311.2 | 01:57.071 | 1.034 / 0.019 | |||
| 19 | Alex Marquez | Repsol Honda | 312.1 | 01:57.335 | 1.298 / 0.264 | |||
| 20 | Stefan Bradl* | Repsol Honda | 312.1 | 01:57.509 | 1.472 / 0.174 | |||
| 21 | Iker Lecuona | KTM Tech 3 | 310.3 | 01:57.569 | 1.532 / 0.060 |
*Replaced Marc Marquez for the Czech Republic Grand Prix
Note: Pramac Racing rider Francesco Bagnaia was ruled out of the Czech Republic Grand Prix after sustaining a right knee injury in FP1
Repsol Honda rider Marc Marquez was ruled out of the Czech Republic Grand Prix after undergoing a second surgery on a fractured humerus
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