MotoGP Second Free Practice Results Assen Netherlands Saturday June 29
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Francesco Bagnaia | Ducati Lenovo | 313.0 | 01:31.670 | |
| 2 | Maverick Viñales | Aprilia Racing | 308.3 | 01:31.743 | 0.073 / 0.073 |
| 3 | Pedro Acosta | GASGAS Tech3 | 313.8 | 01:31.991 | 0.321 / 0.248 |
| 4 | Marc Marquez | Gresini Racing | 307.5 | 01:32.050 | 0.380 / 0.059 |
| 5 | Jorge Martin | Pramac Racing | 309.8 | 01:32.174 | 0.504 / 0.124 |
| 6 | Franco Morbidelli | Pramac Racing | 309.8 | 01:32.279 | 0.609 / 0.105 |
| 7 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 306.7 | 01:32.318 | 0.648 / 0.039 |
| 8 | Alex Marquez | Gresini Racing | 309.8 | 01:32.327 | 0.657 / 0.009 |
| 9 | Raul Fernandez | Trackhouse Racing | 305.2 | 01:32.407 | 0.737 / 0.080 |
| 10 | Aleix Espargaro | Aprilia Racing | 306.0 | 01:32.411 | 0.741 / 0.004 |
| 11 | Fabio Quartararo | Monster Energy Yamaha | 303.7 | 01:32.446 | 0.776 / 0.035 |
| 12 | Jack Miller | Red Bull KTM | 316.2 | 01:32.486 | 0.816 / 0.040 |
| 13 | Enea Bastianini | Ducati Lenovo | 310.6 | 01:32.489 | 0.819 / 0.003 |
| 14 | Marco Bezzecchi | Pertamina Enduro VR46 | 309.8 | 01:32.670 | 1.000 / 0.181 |
| 15 | Brad Binder | Red Bull KTM | 310.6 | 01:32.690 | 1.020 / 0.020 |
| 16 | Miguel Oliveira | Trackhouse Racing | 310.6 | 01:32.766 | 1.096 / 0.076 |
| 17 | Joan Mir | Repsol Honda | 306.0 | 01:32.844 | 1.174 / 0.078 |
| 18 | Alex Rins | Monster Energy Yamaha | 306.0 | 01:33.042 | 1.372 / 0.198 |
| 19 | Johann Zarco | LCR Honda Castrol | 308.3 | 01:33.244 | 1.574 / 0.202 |
| 20 | Lorenzo Savadori* | Aprilia Racing | 308.3 | 01:33.388 | 1.718 / 0.144 |
| 21 | Takaaki Nakagami | LCR Honda Idemitsu | 305.2 | 01:33.396 | 1.726 / 0.008 |
| 22 | Augusto Fernandez | GASGAS Tech3 | 304.4 | 01:33.410 | 1.740 / 0.014 |
| 23 | Luca Marini | Repsol Honda | 308.3 | 01:33.468 | 1.798 / 0.058 |
*Wild card entry