MotoGP Second Free Practice Results Mugello Italy Saturday June 1
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Francesco Bagnaia | Ducati Lenovo | 357.6 | 01:45.608 | |
| 2 | Marc Marquez | Gresini Racing | 360.0 | 01:45.947 | 0.339 / 0.339 |
| 3 | Franco Morbidelli | Pramac Racing | 357.6 | 01:46.036 | 0.428 / 0.089 |
| 4 | Enea Bastianini | Ducati Lenovo | 357.6 | 01:46.084 | 0.476 / 0.048 |
| 5 | Pedro Acosta | GASGAS Tech3 | 360.0 | 01:46.099 | 0.491 / 0.015 |
| 6 | Maverick Viñales | Aprilia Racing | 358.8 | 01:46.100 | 0.492 / 0.001 |
| 7 | Fabio Quartararo | Monster Energy Yamaha | 357.6 | 01:46.260 | 0.652 / 0.160 |
| 8 | Brad Binder | Red Bull KTM | 360.0 | 01:46.273 | 0.665 / 0.013 |
| 9 | Alex Marquez | Gresini Racing | 354.0 | 01:46.284 | 0.676 / 0.011 |
| 10 | Marco Bezzecchi | Pertamina Enduro VR46 | 352.9 | 01:46.304 | 0.696 / 0.020 |
| 11 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 355.2 | 01:46.368 | 0.760 / 0.064 |
| 12 | Aleix Espargaro | Aprilia Racing | 352.9 | 01:46.430 | 0.822 / 0.062 |
| 13 | Raul Fernandez | Trackhouse Racing | 348.3 | 01:46.476 | 0.868 / 0.046 |
| 14 | Jack Miller | Red Bull KTM | 362.4 | 01:46.498 | 0.890 / 0.022 |
| 15 | Jorge Martin | Pramac Racing | 356.4 | 01:46.549 | 0.941 / 0.051 |
| 16 | Pol Espargaro* | Red Bull KTM | 362.4 | 01:46.634 | 1.026 / 0.085 |
| 17 | Alex Rins | Monster Energy Yamaha | 352.9 | 01:46.657 | 1.049 / 0.023 |
| 18 | Miguel Oliveira | Trackhouse Racing | 355.2 | 01:46.700 | 1.092 / 0.043 |
| 19 | Augusto Fernandez | GASGAS Tech3 | 357.6 | 01:46.856 | 1.248 / 0.156 |
| 20 | Takaaki Nakagami | LCR Honda Idemitsu | 351.7 | 01:47.099 | 1.491 / 0.243 |
| 21 | Johann Zarco | LCR Honda Castrol | 357.6 | 01:47.213 | 1.605 / 0.114 |
| 22 | Joan Mir | Repsol Honda | 355.2 | 01:47.245 | 1.637 / 0.032 |
| 23 | Lorenzo Savadori* | Aprilia Racing | 349.5 | 01:47.416 | 1.808 / 0.171 |
| 24 | Luca Marini | Repsol Honda | 356.4 | 01:47.468 | 1.860 / 0.052 |
*Wild card entry (2)