MotoGP Practice Results Misano Italy Friday September 20
| P | Rider | Team | Km/h | Lap time | Gap 1st/Prev. |
|---|---|---|---|---|---|
| 1 | Francesco Bagnaia | Ducati Lenovo | 299.1 | 01:30.286 | |
| 2 | Jorge Martin | Pramac Racing | 299.1 | 01:30.484 | 0.198 / 0.198 |
| 3 | Marc Marquez | Gresini Racing | 298.3 | 01:30.585 | 0.299 / 0.101 |
| 4 | Enea Bastianini | Ducati Lenovo | 300.0 | 01:30.607 | 0.321 / 0.022 |
| 5 | Fabio Quartararo | Monster Energy Yamaha | 294.2 | 01:30.886 | 0.600 / 0.279 |
| 6 | Franco Morbidelli | Pramac Racing | 298.3 | 01:30.932 | 0.646 / 0.046 |
| 7 | Marco Bezzecchi | Pertamina Enduro VR46 | 299.1 | 01:30.964 | 0.678 / 0.032 |
| 8 | Maverick Viñales | Aprilia Racing | 297.5 | 01:30.990 | 0.704 / 0.026 |
| 9 | Pedro Acosta | GASGAS Tech3 | 299.1 | 01:30.991 | 0.705 / 0.001 |
| 10 | Aleix Espargaro | Aprilia Racing | 295.8 | 01:31.167 | 0.881 / 0.176 |
| 11 | Jack Miller | Red Bull KTM | 297.5 | 01:31.217 | 0.931 / 0.050 |
| 12 | Johann Zarco | LCR Honda Castrol | 297.5 | 01:31.250 | 0.964 / 0.033 |
| 13 | Fabio Di Giannantonio | Pertamina Enduro VR46 | 293.4 | 01:31.393 | 1.107 / 0.143 |
| 14 | Alex Marquez | Gresini Racing | 296.7 | 01:31.513 | 1.227 / 0.120 |
| 15 | Brad Binder | Red Bull KTM | 298.3 | 01:31.648 | 1.362 / 0.135 |
| 16 | Miguel Oliveira | Trackhouse Racing | 298.3 | 01:31.668 | 1.382 / 0.020 |
| 17 | Raul Fernandez | Trackhouse Racing | 295.8 | 01:31.720 | 1.434 / 0.052 |
| 18 | Luca Marini | Repsol Honda | 296.7 | 01:31.765 | 1.479 / 0.045 |
| 19 | Joan Mir | Repsol Honda | 300.8 | 01:31.924 | 1.638 / 0.159 |
| 20 | Augusto Fernandez | GASGAS Tech3 | 297.5 | 01:32.100 | 1.814 / 0.176 |
| 21 | Takaaki Nakagami | LCR Honda Idemitsu | 295.8 | 01:32.210 | 1.924 / 0.110 |
Note: Monster Energy Yamaha rider Alex Rins did not take part in Practice due to fever.